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KMID : 0829220140380030139
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Korean Journal of Oral and Maxillofacial Pathology
2014 Volume.38 No. 3 p.139 ~ p.146
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Effect of Osteogenic Differentiation by Fucoidan in Human Alveolar Bone Marrow-derived Mesenchymal Stem Cells
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김범수:Kim Beom-Su
강효진:Kang Hyo-Jin/양선식:Yang Sun-Sik/윤정훈:Yoon Jung-Hoon/이준:Lee Jun/박지윤:Park Ji-Yun
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Abstract
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Fucoidan has been extensively studied as medicinal materials due to its biological activities including osteoblastic differentiation effect. However, osteoblastic effect by fucoidan is unknown in alveolar bone marrow derived mesenchymal stem cells (ABM-MSCs). The present study was undertaken to evaluate the effect of fucoidan on Osteoblastic differentiation in ABM-MSCs and explore its mechanism. Cell proliferation was analyzed by crystal violet staining. Osteoblast differentiation was determined by alkaline phosphatase activity, calcium accumulation assay and gene expression of osteoblast markers. We found that fucoidan induced cell proliferation of ABM-MSCs. Furthermore, fucoidan increased the ALP activity, calcium accumulation, and osteoblast specific genes such as Runx2, type I collagen alpha 1. Moreover, fucoidan induces the expression of asporin and bone morphogenic protein (BMP)-2 and asporin. Based on these results, these finding indicate that fucoidan induces osteoblast differentiation in ABM-MSCs and partially enhanced the mRNA expression of BMP-2 and asporin.
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KEYWORD
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Fucoidan, Alveolar bone marrow-mesenchymal stem cells, Osteoblast dfferentiation, BMP-2, Asporin
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